e-Science logo Nesc logo
 
 
About NeSC
e-Science Institute
e-Science Hub
TOE
Contacts
e-Science Events
Resources
Newsroom
Presentations & Lectures
Technical Papers
Global Grid Links
Projects
UK e-Science Centres
UK e-Science Teams
Career Opportunities
Bibliographic Database
 

 

Paper ID: 2737

PEF-1, an epithelial cell transcription factor which activates the long control region of human papillomavirus type 16, is glycosylated with N- acetylglucosamine
D Fergusson and MS Campo,

Appeared in: Journal of General Biology
Page Numbers:2753-2760
Publisher: N/A
Year: 1998
ISBN/ISSN:
Contributing Organisation(s): The Beatson Institute for Cancer Research
Field of Science: Medicine

URL: http://carlin.lib.ed.ac.uk:2396/cgi/reprint/79/11/2753

Abstract: Infection by human papillomavirus type 16 (HPV-16) has been linked to cervical cancer. The transcription of viral genes in HPV-16 is partially controlled by a number of cellular transcription factors. We have previously identified a novel cellular transcription factor, PEF- 1, from its ability to interact with the long control region (LCR) of HPV-16. This factor has a molecular mass of about 110 kDa and binds to a GC-rich sequence in the section of the LCR responsible for cell type- specific transcription from viral DNA. The factor Sp1 has similar properties and also interacts with the HPV-16 LCR. We show that PEF-1 and Sp1 are distinct transcription factors: they recognize different DNA sequences, have different electrophoretic mobilities and different glycosylation patterns. Sp1 is O-glycosylated while PEF-1 appears to have a novel type of glycosylation, as shown by the interaction with pokeweed lectin and by the inhibition of this interaction by tunicamycin.

Keywords:


BIB DOC HTM HTML PDF PPT PS RTF TEX TXT ZIP




 

Last Updated: 22 Jun 12 11:02
This is an archived website, preserved and hosted by the School of Physics and Astronomy at the University of Edinburgh. The School of Physics and Astronomy takes no responsibility for the content, accuracy or freshness of this website. Please email webmaster [at] ph [dot] ed [dot] ac [dot] uk for enquiries about this archive.